Proteus Metal Chelate kits are ideal for screening expression clones for high levels of 6xHis-tagged proteins, scouting mutant clones for recombinant protein expression and multiple small-scale and preparative purification of 6xHis-tagged proteins for activity and structural studies.
 

Device

IMAC Mini

IMAC Midi

Max. sample volume per load

0.65 ml, fixed angle rotor

20 ml, fixed angle rotor

Charged metal ion

Ni2+

Ni2+

Typical binding capacity per use

1 mg 6xHis-tagged protein

10 mg 6xHis-tagged protein

Min. number of uses per plug

2 (assuming no further Ni2+ charging)

2 (assuming no further Ni2+ charging)

Supporting Proteus matrix

Covalently coupled to agarose resin

Covalently coupled to agarose resin

Resin bed volume

0.23 ml

1.6 ml

Bead size range

45-165 µm

45-165 µm

Storage temperature for resin plugs

2-8 °C

2-8 °C

Recommended working pH

pH 2.0-12.0

pH 2.0-12.0

Chemical stability

High

High

Plastic construction

Polypropylene

Polypropylene

Time to purity

15 min

48 min

Colour coded end caps

Black

Black