Proteus purification kits incorporate patented, single step and affinity spin columns, clear and unambiguous procedures, desalting/buffer exchange columns and ready-to-use buffers.

Proteus Metal Chelate kits are ideal for screening expression clones for high levels of 6xHis-tagged proteins, scouting mutant clones for recombinant protein expression and multiple small-scale and preparative purification of 6xHis-tagged proteins for activity and structural studies.

Convenient & easy-to-use
• Simply insert the pre-packed chromatography resin plug into the device and place it in the centrifuge.
• No mess, no filling columns, no attachment of accessories, no pumps and no lengthy equilibration.
• Kits are supplied with a comprehensive technical and application-supported handbook, a stepwise laminated protocol card and all necessary buffers for rapid and simple recombinant protein purification.

Rapid
• The purification protocols provided in the operating handbook permit fast recovery of pure recombinant protein.
• Multiple samples can be processed at the same time, allowing rapid screening for 6xHis-tagged proteins.
• No hold-up volume or spluttering. High recovery during the fast elution step.

Cost-effective
• Proteus spin columns can be used by any laboratory with access to a centrifuge.
• The Proteus columns are re-usable. Each Mini spin column can be used up to a minimum of 2 times and each Midi spin column can be used up to a minimum of 2 times without nickel re-charging.
• As research budgets are always under pressure, Proteus kits offer a cost-effective alternative.

Performance
• Proteus chromatography spin columns will typically bind the target recombinant protein with greater than 90% recovery.
• Proteus spin columns can purify and concentrate the protein of interest in a single step and with high reproducibility (low % CV).
• Proteins are sufficiently pure for structural and activity-based studies or for raising antibodies.