|
The Mini Protein A & G spin column is the ideal tool for screening antibody expression and for small-scale purification of antibodies for solution-state immuno-assays, immuno-histochemical and immuno-fluorescence studies, Western blotting and immuno-precipitation studies. Antibodies are purified using a powerful, patented affinity spin column using a microfuge common to all biochemistry and immunology laboratories.
|
Mini Device | Protein A | Protein G |
Source | Recombinant Protein A expressed in E. coli (N.B. No toxic bacterial contaminants normally found in native Protein A). | Recombinant Protein G expressed in E. coli (N.B. The recombinant Protein G lacks the albumin-binding domain found in native Protein G). |
 |
Binding capacity | Typically 1 mg human IgG | Typically 1 mg human IgG |
|
Typical number of uses per plug | 3 | 3 |
|
Supporting Proteus matrix | Covalently coupled to agarose resin | Covalently coupled to agarose resin |
|
Resin bed volume | 0.23 ml | 0.23 ml |
|
Ligand density | 3.5 mg Protein A/ml resin | 2 mg Protein G/ml resin |
|
Bead size range | 60-165 µm | 45-165 µm |
|
Maximum volume | 0.65 ml | 0.65 ml |
|
Recommended working pH | pH 2.5-9.0 | pH 2.5-9.0 |
|
Storage temperature for resin plugs | 2-8 °C | 2-8 °C |
|
Plastic construction | Polypropylene | Polypropylene |
|
Colour coded end caps | Yellow | Red |
|
The Midi Protein A & G spin column permits semi-preparative purification of concentrated monoclonal and polyclonal antibodies for all down stream applications. Both the Mini and Midi kits contain all the resin spin columns, buffers and ultrafiltration spinners necessary for rapid and convenient purifications of your target antibodies.
|
Midi Device | Protein A | Protein G |
Source | Recombinant Protein A expressed in E. coli (N.B. No toxic bacterial contaminants normally found in native Protein A). | Recombinant Protein G expressed in E. coli (N.B. The recombinant Protein G lacks the albumin-binding domain found in native Protein G). |
|
Binding capacity | Typically 20 mg human IgG | Typically 20 mg human IgG |
|
Typical number of uses per plug | 5 | 5 |
|
Supporting Proteus matrix | Covalently coupled to agarose resin | Covalently coupled to agarose resin |
|
Resin bed volume | 1.6 ml | 1.6 ml |
|
Ligand density | 3.5 mg Protein A/ml resin | 2 mg Protein G/ml resin |
|
Bead size range | 60-165 µm | 45-165 µm |
|
Maximum volume | 20 ml | 20 ml |
|
Recommended working pH | pH 2.5-9.0 | pH 2.5-9.0 |
|
Storage temperature for resin plugs | 2-8 °C | 2-8 °C |
|
Plastic construction | Polypropylene | Polypropylene |
|
Colour coded end caps | Yellow | Red |
|
|
|
